sortase a srta plasmid Search Results


sortase a esrta pentamutant  (Addgene inc)
94
Addgene inc sortase a esrta pentamutant
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sortase A Esrta Pentamutant, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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his sortase a 7m  (Addgene inc)
93
Addgene inc his sortase a 7m
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
His Sortase A 7m, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sortase a heptamutant  (Addgene inc)
93
Addgene inc sortase a heptamutant
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sortase A Heptamutant, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sortase a pentamutant  (Addgene inc)
93
Addgene inc sortase a pentamutant
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sortase A Pentamutant, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sortase a mediated conjugation  (Addgene inc)
91
Addgene inc sortase a mediated conjugation
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sortase A Mediated Conjugation, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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purified sortase a  (Addgene inc)
90
Addgene inc purified sortase a
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Purified Sortase A, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sortase a srta plasmid #75144  (Addgene inc)
90
Addgene inc sortase a srta plasmid #75144
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sortase A Srta Plasmid #75144, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sortase a  (Addgene inc)
90
Addgene inc sortase a
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sortase A, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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staphylococcus aureus sortase a 7 mutant  (Addgene inc)
93
Addgene inc staphylococcus aureus sortase a 7 mutant
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Staphylococcus Aureus Sortase A 7 Mutant, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ca 2 independent sortase a  (Addgene inc)
93
Addgene inc ca 2 independent sortase a
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Ca 2 Independent Sortase A, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid #51140  (Addgene inc)
90
Addgene inc plasmid #51140
Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via <t>eSrtA,</t> while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Plasmid #51140, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via eSrtA, while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: International journal of biological macromolecules

Article Title: Targeting HER2 and FGFR-positive cancer cells with a bispecific cytotoxic conjugate combining anti-HER2 Affibody and FGF2.

doi: 10.1016/j.ijbiomac.2023.127657

Figure Lengend Snippet: Fig. 5. Synthesis of protein-drug conjugates. A) The structure of the cytotoxic payload for sortase A-mediated ligation. The cytotoxic payload (vcMMAE) comprises monomethyl auristatin E (MMAE, pink), a highly potent inhibitor of microtubule metabolism, enriched with a self-immolation p-aminobenzylcarbamate moiety and the L-Val-L-Cit dipeptide that undergoes hydrolysis by lysosomal cathepsin B. The maleimide derivative of vcMMAE is coupled with the G3-PEG12 moiety (purple). The G3 motif enables ligation via eSrtA, while the PEG12 spacer ensures flexibility and appropriate exposition of the payload. B) Synthesis scheme of protein-drug conjugates via eSrtA-mediated ligation. The protein containing the C-terminal LPETGG motif is synthesized with the cytotoxic payload enriched with the N-terminal GGG motif. C) Purity analysis of the conjugates confirmed by SDS-PAGE. D) UV–Vis spectra for calculation of drug-to-protein ratio (DPR). The DPR values are provided in the figure legend. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Evolved sortase A (eSrtA) pentamutant with improved kinetics and activity in the pET29 plasmid was a gift from David Liu (Addgene plasmid # 75144) [38].

Techniques: Ligation, Synthesized, SDS Page